File Name: exosome-mediated delivery of sirna in vitro and in vivo writer.zip
Voltage between 0. B Different amounts of MSC exosomes containing 0. The exosomal protein concentrations of 0. Exosomes, widely recognized natural nanovesicles, represent one of the recently discovered modes of intercellular communication due to their ability to transmit crucial cellular information that can be engineered to have robust delivery and targeting capacity. MiRp, one of the upregulated microRNAs miRNAs in many types of breast cancer, activates the canonical Wnt signaling pathway and transactivates the miR expression, and results in the hyperproliferation of cancer cells in vitro and mammary glands in vivo. In this study, we exploited the exosomes isolated from bone marrow-derived mesenchymal stem cells MSCs-Exo to deliver LNA locked nucleic acid -modified anti-miRp oligonucleotides to suppress the expression level of miRp and miR in 4T1 and TUBO breast cancer cell lines.
Lack of effective tumor-specific delivery systems remains an unmet clinical challenge for successful translation of innovative therapies, such as, therapeutic oligonucleotides. In the past decade, exosomes have been suggested to be ideal drug delivery systems with application in a broad range of pathologies including cancer, due to their organotropic properties. Tumor-derived exosomes, having tumor-homing properties, can efficiently reach cancer cells and therefore behave as carriers for improved drug delivery to the primary tumor and metastases. However, due to their complex composition, and still undefined biological functions, safety concerns arise hampering their translation to the clinics. We propose here the development of exosome-mimetic nanosystems EMNs that simulate natural tumor-derived exosomes with respect to their structure and functionality, but with a controlled composition, for the targeted delivery of therapeutic oligonucleotides to lung adenocarcinoma cells microRNA mimics.
Exosomes are small, cell-secreted vesicles that transfer proteins and genetic information between cells. This intercellular transmission regulates many physiological and pathological processes. Therefore, exosomes have emerged as novel biomarkers for disease diagnosis and as nanocarriers for drug delivery. Here, we report an easy-to-adapt and highly versatile methodology to modulate exosome composition and conjugate exosomes for intracellular delivery. The azide-integrated can be conjugated to a variety of small molecules and proteins and can efficiently deliver conjugates into cells.
In the s an unexpected gene-silencing phenomena in plants, the later called RNA interference RNAi , perplexed scientists. Following the proof of activity in mammalian cells, small interfering RNAs siRNAs have quickly crept into biomedical research as a new powerful tool for the potential treatment of different human diseases based on altered gene expression. In the past decades, several promising data from ongoing clinical trials have been reported.
Living with art getlein pdf thousand miles piano sheet music pdf freeReply