peptide characterization and application protocols pdf

Peptide characterization and application protocols pdf

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Peptide Separation

Applications & Protocols

HPLC Analysis and Purification of Peptides

Peptide Separation

Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Popa, James M. Kovacs, Janine B. Mills, Brian P. Tripet, and Robert S.

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The peptide competition assay PCA is a recommended procedure for confirming the specific band reactivity of antipeptide polyclonal antibodies, especially domain specific antibodies like phospho-specific antibodies. It is not uncommon to see more than one band on immunoblots of lysates when probing with a primary antibody. The PCA provides a means of determining which band or staining pattern is specific for the antibody. Typically the experiment should be run in parallel; once with the phosphorylated form of the peptide immunogen and once with the non-phosphorylated form of the immunizing peptide. In the PCA, the antibody is pre-incubated with the peptide or peptides prior to use in immunoblotting assays.

Applications & Protocols

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Go back to menu. Correlation about 0. Petrilli et al. Higgins Columns Part Numbers and Prices. Importance of TFA Concentration for a reproducible peptide separation. Hao, et al. Proteome Res.


Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical.


HPLC Analysis and Purification of Peptides

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography HPLC for purification and evaluation of peptides and mass spectrometry MS for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides.

Table of contents

The SCX stationary phase usually contains aliphatic sulfonic acid groups that are negatively charged in aqueous solution, therefore tightly binding any strongly basic analytes. To recover the analyte, the resin is then washed with a solvent neutralizing this ionic interaction. SCX fractionation can also be performed in a solid-phase extraction cartridge format for a rapid but lower resolution fractionation. SCX can also be conducted in microscale solid-phase extraction format i. Fractionation of peptides by strong cation-exchange liquid chromatography.

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    Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills et al. Pages PDF.

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