File Name: coding and noncoding sequences ppt to .zip
Other functions of non-coding DNA include the transcriptional and translational regulation of protein-coding sequences, scaffold attachment regions , origins of DNA replication , centromeres and telomeres. The amount of non-coding DNA varies greatly among species. Often, only a small percentage of the genome is responsible for coding proteins, but an increasing percentage is being shown to have regulatory functions. When there is much non-coding DNA, a large proportion appears to have no biological function, as predicted in the s. Since that time, this non-functional portion has controversially been called "junk DNA". The amount of total genomic DNA varies widely between organisms, and the proportion of coding and non-coding DNA within these genomes varies greatly as well.
Comparative sequence analysis has facilitated the discovery of protein coding genes and important functional sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of sequences of relatively closely related species. We tested the use of comparative DNA sequence analysis to aid identification of promoter regulatory elements, nonprotein-coding RNA genes, and small protein-coding genes by surveying random DNA sequences of several Saccharomyces yeast species, with the goal of learning which species are best suited for comparisons with S. We also determined the DNA sequence of a few specific promoters and RNA genes of several Saccharomyces species to determine the degree of conservation of known functional elements within the genome. Our results lead us to conclude that comparative DNA sequence analysis will enable identification of functionally conserved elements within the yeast genome, and suggest a path for obtaining this information. Identifying functional elements in DNA sequence is a significant challenge.
Finding DNA sequences that show significant signs of positive or negative selection Regulatory regions ~ conserved non-coding sequences. ▫ Pennachio etc.
Metrics details. Retrotransposons are key players in the evolution of eukaryotic genomes. Moreover, it is now known that some retrotransposon classes, like the abundant and plant-specific Sireviruses, have intriguingly distinctive host preferences.
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The comparison of complete genomes has revealed surprisingly large numbers of conserved non-protein-coding CNC DNA regions.Reply
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